Department of Biophysics and Biophysical Chemistry
School of Medicine
B.S. 1990, Swarthmore College
Ph.D. 1995, Harvard University
725 N. Wolfe Street
Baltimore, MD 21205
In eukaryotes, assembly of a ribosome on an mRNA (translation initiation) requires the action of at least 12 initiation factors and energy input from the hydrolysis of both ATP and GTP. Translation initiation is a key point in the control of gene expression and breakdown of proper initiation and its control play roles in a number of human diseases. Our lab uses a fully reconstituted yeast-based translation initiation system and a wide variety of biochemical and biophysical methods to dissect the molecular mechanics of this central biological process. We collaborate closely with geneticists and structural biologists in order to build a complete picture of the operation of the eukaryotic translational machinery. We are also developing chemical reagents to control translation initiation, with the hope that they may some day become drugs.
Mitchell, S.F., S.E. Walker, M.A. Algire, E.H. Park, A.G. Hinnebusch, and J.R. Lorsch. (2010) The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway. Mol. Cell 39:950-962.
Saini, A.K., J.S. Nanda, J.R. Lorsch, and A.G. Hinnebusch. (2010) Regulatory elements in eIF1A control the fidelity of start codon selection by modulating tRNA(i)(Met) binding to the ribosome. Genes Dev. 24:97-110.
Nanda, J.S., Y.N. Cheung, J.E. Takacs, P. Martin-Marcos, A.K. Saini, A.G. Hinnebusch, and J.R. Lorsch. (2009) eIF1 controls multiple steps in start codon recognition during eukaryotic translation initiation. J. Mol. Biol. Sep 12. [Epub ahead of print] PMID: 19751744
Kolitz, S.E., J.E. Takacs, and J.R. Lorsch. (2009) Kinetic and thermodynamic analysis of the role of start codon/anticodon base pairing during eukaryotic translation initiation. RNA 15:138-152.
Acker, M.G., B.S. Shin, J.S. Nanda, A.K. Saini, T.E. Dever, and J.R. Lorsch. (2009) Kinetic analysis of late steps of eukaryotic translation initiation. J. Mol. Biol. 385:491-506.
Cheung, Y.N., D. Maag, S.F. Mitchell, C.A. Fekete, M.A. Algire, J.E. Takacs, N. Shirokikh, T. Pestova, J.R. Lorsch, and A.G. Hinnebusch. (2007) Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo. Genes Dev. 21:1217-30.
Passmore, L.A., T.M. Schmeing, D. Maag, D.J. Applefield, M.G. Acker, M.A. Algire, J.R. Lorsch, and V. Ramakrishnan. (2007) The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome. Mol. Cell 26:41-50.
Fekete, C.A., S.F. Mitchell, V.A. Cherkasova, D. Applefield, M.A. Algire, D. Maag, A.K. Saini, J.R. Lorsch, and A.G. Hinnebusch. (2007) N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection. EMBO J. 26:1602-14.
Kapp, L.D., S.E. Kolitz, and J.R. Lorsch. (2006) Yeast initiator tRNA identity elements cooperate to influence multiple steps of translation initiation. RNA 12:751-64.
Acker, M.G., B.S. Shin, T.E. Dever, and J.R. Lorsch. (2006) Interaction between eukaryotic initiation factors 1A and 5B is required for efficient ribosomal subunit joining. J. Biol. Chem. 281:8469-8475.
Maag, D., M.A. Algire, and J.R. Lorsch. (2006) Communication between eukaryotic translation initiation factors 5 and 1A within the ribosomal pre-initiation complex plays a role in start site selection. J. Mol. Biol. 356:724-737.
Algire, M.A., D. Maag, and J.R. Lorsch. (2005) Pi release from eIF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation. Mol. Cell 20:251-262.
Fekete, C.A., D.J. Applefield, S.A. Blakely, N. Shirokikh, T. Pestova, J.R. Lorsch, and A.G. Hinnebusch. (2005) The eIF1A C-terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo. EMBO J. 24:3588-3601.
Maag, D., C.A. Fekete, Z. Gryczynski and J.R. Lorsch. (2005) A conformational change in the eukaryotic translation preinitiation complex and release of elF1 signal recognition of the start codon. Mol. Cell 17:265-275.